Laboratory methods - laboratory classes
Osnova sekce
-
In this section, you will find all the materials you need for the Biochemistry I laboratory course.
Laboratory classes:
- Laboratory Practise and Safety Rules. Introduction to Spectrophotometry
- Electrochemical methods – Potentiometry
- Optical methods – Spectrophotometry
- The pH measurement, buffers
- Factors influencing laboratory results
- Chromatographic methods
- Properties of amino acids and proteins
- Kinetics of enzymatic reactions
- Properties of enzymes
Application of knowledge related to the topic of the seminar:
- General Medicine – Biochemistry exam questions
- Chromatography and its usage in medicine
- Possible preanalytical and analytical errors during analysis of blood, serum or urine
- Principles of electrophoretic separation of plasma proteins, diagnostic importance
- Principles of spectrophotometric analysis of basic analytes from serum
Download the Labbook below.
Don't forget to bring a printed labbook to each lab class!
-
BEFORE THE LABORATORY CLASS:
- Read the safety rules and complete the test at 100%
- Watch the video lesson - Laboratory glassware
- Watch the video lesson - Rules of good pipetting practice
- Study the microlesson to spectrophotometry
- Print the LABBOOK!
THE AIMS OF LABORATORY CLASS:
- Learn the Safety rules in the laboratory
- Learn the basic pipetting
- Understanding the basic principles of absorption spectrophotometry
- Understanding the basic fact of UV/VIS spectrophotometry
EXAMINATION TOPICS:
- Bases of UV/VIS spectrophotometry
- The Transmittance, the absorbance, the Lambert-Beer law
-
-
This test is OBLIGATORY, without passing it you will not be allowed to take practical lessons. You have unlimited number attempt, we recommend read the safety rules before completing this test. The required output is a 100% success rate.
-
Here you will find the theoretical materials to study on the topic of Optical Analytical Methods.
-
BEFORE THE LABORATORY CLASS:
- Repeat the introduction to spectrophotometry (1st laboratory class) and study Labmbert-Beer law
- Complete the mandatory test at 80% (we recommend the test for practicing with an unlimited number of attempts)
- Watch the video lesson to spectrophotometry
THE AIMS OF LABORATORY CLASS:
- Understanding the basic facts about UV/VIS spectrophotometry
- Learn the methods of calculating the concentration from the obtained absorbance value
EXAMINATION TOPICS:
- basic optical methods
- bases of UV/VIS spectrophotometry
- the Lambert-Beer law
- how to determine the concentration of a compound in solution
BRING THE PRINTED PROTOCOL - LABBOOK!
-
Here you will find the theoretical materials to study on the topic of Methods of Calculating Unknown Concentration – Spectrophotometry.
-
-
The test is written at the beginning of the practical exercises. To successfully pass the test, 4 out of 5 answers must be correct.
-
AIM:
- Master the calibration of the pH-meter
- Perform alkalimetric titration by means of potentiometry
- Construct a plot of pH dependence on the added amount of NaOH
- Characterize titration curve of acetic acid titrated with NaOH
EXAMINED TOPICS:
- basic electrochemical methods (potenciometry, types of electrodes – standard and indicatory electrodes – neutralisation titration, redox titration, precipitation titration, equivalence point)
- weak acids and bases, pH calculation, dissociation constan
- buffers – basic characteristics, acetate buffer – titration curve, buffer capacity
BRING THE PRINTED PROTOCOL - LABBOOK!-
Here you will find the theoretical materials to study on the topic of Electrochemical methods.
-
-
-
The test is written at the beginning of the practical exercises. To successfully pass the test, 4 out of 5 answers must be correct.
-
AIM:
- Verify theoretical knowledge about pH and its measurements
- Verify the properties of buffers
EXAMINED TOPICS:
- basic properties of buffers
- how to measure pH of buffers (universal pH-papers, pHan papers, pH-meters)
- buffers in biological systems
- bicarbonate buffer in body – pH calculation
- determination of HCO3- concentration (medical purposes)
BRING THE PRINTED PROTOCOL - LABBOOK!
-
THE AIMS OF LABORATORY CLASS:
- Understanding the individual's contribution to the occurrence of errors in the analytical phase of measurement
- Improving pipetting
- Practicing the different ways of sample dilution
THE EXAMINATION TOPICS:
- praeanalytical, analytical and postanalytical errors
- precision and accuracy of methods
- dilution of solution – calculations
- how to dilute specimens in laboratory
- blank“ sample in spectrophotometry
BRING THE PRINTED PROTOCOL - LABBOOK!
-
Here you will find the theoretical materials to study on the topic of Factors influencing laboratory results.
-
Studenti musíZobrazit522.2 KB
-
-
Studenti musíZískat nějakou známku
The test must be completed before the start of the laboratory practice at 100%. You have 5 attempts to test.
-
On-line laboratory classes
As part of the laboratory class on chromatographic methods, the following tasks must be completed:
-
Study the provided materials related to the topic.
-
Complete and upload the worksheet (Case Report) in this category, under the folder “Worksheet upload – Chromatography.”
-
Write your answers directly into the worksheet.
-
Don’t forget to sign the worksheet and indicate your group — ideally include this information directly in the file name when uploading.
-
-
Successfully complete the final test (5 questions – 4 correct answers are required to pass).
All tasks must be completed and submitted no later than Friday (October 31) by 8:00 p.m.
THE AIMS OF A CLASS:
- Understanding the basic principles of liquid chromatography and mass spectrometry and their use in clinical practice
- Case report: Evaluation of chromatogram, identifying compounds responsible for patient intoxication and assesment of plasmatic concentrations of these compounds
EXAMINATION TOPICS:
- Basic principle of LC-MS
- Example uses of this technique in clinical practice and medical research
-
Here you will find the theoretical materials to study on the topic of Chromatographic methods.
-
-
-
-
Studenti musíZískat nějakou známkuZískat požadovanou známkuZískat potřebnou známku nebo dokončit všechny dostupné pokusy
To complete during lab class, one attempt only.
-
In case of any questions please use this discussion forum.
-
Studenti musíOznačit jako hotovo
-
-
On-line laboratory classes
As part of the laboratory class on Properties of aminoacids and proteins, the following tasks must be completed:
-
Study the provided materials related to the topic.
-
Complete and upload the worksheet in this category, under the folder “Worksheet upload”
-
Write your answers directly into the worksheet.
-
Don’t forget to sign the worksheet and indicate your group — ideally include this information directly in the file name when uploading.
-
-
Successfully complete the final test (5 questions – 4 correct answers are required to pass).
All tasks must be completed and submitted no later than November 22th by 8:00 p.m
-
-
Here you will find the theoretical materials to study on the topic of Properties of amino acids and proteins.
-
-
-
-
-
The aim of the laboratory class:
- To learn how to perform experiments on enzymes
- To verify the influence of environment on enzyme activity
Laboratory class – examination topics:
- substrate and kinetic specificity
- influence of pH, temperature, inhibitors on enzyme activity
- pH optimum – importance for digestive enzymes
- decrease and increase in temperature – importance in medicine
- competitive and non-competitive inhibitions
- allosteric inhibition in regulation
BRING THE PRINTED PROTOCOL - LABBOOK!
-
Here you will find the theoretical materials to study on the topic of Properties of enzymes.
-
-
The aim of the laboratory class:
Measure and evaluate the kinetic parameters of the reaction catalysed by lactate dehydrogenase (LD)
From the measured data determine the maximum reaction velocity (Vmax) and the Michaelis constant (Km).
From the measured data determine the activity of lactate dehydrogenase in the serum sample
Laboratory class – examination topics:
- enzyme structure
- kinetics of enzyme reactions, characterisation of the Michaelis constant ( 1st and zero order reactions), the Lineweaver-Burk plot
- how to determine rate of an enzyme reaction (decrease in substrate, increase in product)
- lactate dehydrogenase
Mini-test - 5 questions focused on enzyme properties and kineticsYou can study the topic using test questions and answers in the E-test available in the MOODLE course below
BRING THE PRINTED PROTOCOL - LABBOOK!
-
Here you will find the theoretical materials to study on the topic of Kinetics of enzyme reactions.
-
-
-
Here you will find a general forum for Laboratory Methods – Practical Exercises.
When posting questions, comments, or suggestions, please remember to indicate which practical exercise topic your post refers to.
-