Optical methods – Spectrophotometry

This chapter introduces optical analytical methods, which represent essential tools in clinical biochemistry. These methods are based on the interaction of electromagnetic radiation with a sample and the subsequent measurement of phenomena such as absorption, emission, fluorescence, or light scattering. Particular emphasis is placed on spectrophotometry, as it forms the cornerstone of routine laboratory diagnostics.

Table of contents:

• 2. Optical Methods: General Overview
• 3. Absorption of radiation
• 4. Absorption Methods – Spectrophotometry
• 5. Lambert-Beer law
• 6. Spectrophotometer – Components
• 7. Applications of Spectrophotometry in Medicine
• 8. Fluorescence
• 9. Emission
• 10. Light Scattering
• 11. Summary