Chromatographic methods

Chromatography is one of the most important separation methods in modern analytical chemistry and biochemistry. It enables effective separation, identification, and quantification of components in complex mixtures based on their different interactions with the mobile and stationary phases. In clinical biochemistry and laboratory diagnostics, chromatography has fundamental importance—it is used for the determination of amino acids, vitamins, hormones, drugs, metabolites, and for monitoring disease markers.

Table of contents:

• 2. Introduction to Chromatographic Methods
• 3. How Chromatography Works
• 4. Separation
• 5. Examples of Separation Techniques by Interaction of the Analyte with the Mobile and Stationary Phase
• 6. Selected Chromatographic Techniques (GC and HPLC)
• 7. Detection in Chromatography
• 8. Chromatogram
• 9. Applications of Chromatographic Separation Techniques in Medicine