1. Chapter Overview

In this chapter, we will explore how enzymes work, what distinguishes them from other catalysts, and why they are essential for life. First, we will explain why some reactions proceed slowly and how enzymes lower the activation energy. Next, we will show how physicochemical conditions of the environment (pH, temperature, ionic strength, redox state) and the presence of inhibitors or activators influence their activity. Finally, we will focus on enzyme specificity—the ability of enzymes to recognize only certain substrates or reactions—and demonstrate why this property has fundamental biological as well as pharmacological importance.

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