Optical methods - Spectrophotometry
Osnova sekce
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BEFORE THE LABORATORY CLASS:
- Repeat the introduction to spectrophotometry (1st laboratory class) and study Labmbert-Beer law
- Complete the mandatory test at 80% (we recommend the test for practicing with an unlimited number of attempts)
- Watch the video lesson to spectrophotometry
THE AIMS OF LABORATORY CLASS:
- Understanding the basic facts about UV/VIS spectrophotometry
- Learn the methods of calculating the concentration from the obtained absorbance value
EXAMINATION TOPICS:
- basic optical methods
- bases of UV/VIS spectrophotometry
- the Lambert-Beer law
- how to determine the concentration of a compound in solution
BRING THE PRINTED PROTOCOL - LABBOOK!
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Here you will find the theoretical materials to study on the topic of Methods of Calculating Unknown Concentration – Spectrophotometry.
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The test is written at the beginning of the practical exercises. To successfully pass the test, 4 out of 5 answers must be correct.